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lipofectin reagent  (Thermo Fisher)


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    Thermo Fisher lipofectin reagent
    Lipofectin Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipofectin%C2%AE+reagent/us12357615-1009-10-12?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    lipofectin reagent - by Bioz Stars, 2026-07
    90/100 stars

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    In vitro stability of mRNA vaccine . In vitro stability of gB-RB1B ( left ) and pp38-RB1B ( right ) mRNA molecules was analyzed within 6 to 32 h post-transfection in DF-1 cells. Five hundred thousand cells were seeded per well in a six-well plate in EMEM complete medium (10% FBS+ 1% pen-strep). Cells were transfected with 1 μg mRNA encoding gB and pp38 using Lipofectin for 4 h in the optimum medium (cells in the C+ group were infected with MDV). At 6-, 12- and 32 h post-transfection, cells were washed with PBS and collected following trypsin treatment for RNA extraction. Expression of gB and pp38 was analyzed by real-time PCR.

    Journal: Viruses

    Article Title: A Marek’s Disease Virus Messenger RNA-Based Vaccine Modulates Local and Systemic Immune Responses in Chickens

    doi: 10.3390/v16071156

    Figure Lengend Snippet: In vitro stability of mRNA vaccine . In vitro stability of gB-RB1B ( left ) and pp38-RB1B ( right ) mRNA molecules was analyzed within 6 to 32 h post-transfection in DF-1 cells. Five hundred thousand cells were seeded per well in a six-well plate in EMEM complete medium (10% FBS+ 1% pen-strep). Cells were transfected with 1 μg mRNA encoding gB and pp38 using Lipofectin for 4 h in the optimum medium (cells in the C+ group were infected with MDV). At 6-, 12- and 32 h post-transfection, cells were washed with PBS and collected following trypsin treatment for RNA extraction. Expression of gB and pp38 was analyzed by real-time PCR.

    Article Snippet: When 80% confluent, cells were transfected with 1 μg mRNA encoding gB and pp38 using Lipofectin™ Transfection Reagent (Invitrogen, Carlsbad, CA, USA) for 4 h in optimum media (Thermo Fisher Scientific, USA).

    Techniques: In Vitro, Transfection, Infection, RNA Extraction, Expressing, Real-time Polymerase Chain Reaction